rabbit anti mobp  (Bioss)

Journal: Scientific Reports

Article Title: A single-cell trajectory atlas of striatal development

doi: 10.1038/s41598-023-36255-5

Figure Lengend Snippet: Pseudotime analysis of oligodendrocytes across striatal development and with deletion of Foxp1 . ( A ) Annotated UMAP of oligodendrocyte progenitor cells (OPCs), committed oligodendrocyte precursors (COPs), newly-formed oligodendrocytes (NFOL), myelin-forming oligodendrocytes (MFOL), mature oligodendrocytes (MOL) and progenitor clusters from the combined dataset. ( B ) Cells colored by PHATE pseudotime values. ( C ) Scaled expression of genes specific to each oligodendrocyte subtype. ( D ) Number of differentially expressed genes (DEGs) in OPCs from P9 dataset with deletion of Foxp1 from dSPNs, iSPNs, or both. Filled bars are upregulated DEGs (such as Mobp ) and grey bars are downregulated DEGs. ( E ) Western blot of TUJ1 (housekeeping gene) and MOBP (oligodendrocyte marker) in striatal tissue in Foxp1 CTL , Foxp1 D1 , Foxp1 D2 , and Foxp1 DD mice and ( F ) quantification of MOBP levels relative to TUJ1 across genotypes (N = 5/genotype). Data is represented as mean ± SEM. P-values determined using a one-way ANOVA with Dunnett’s multiple comparison.

Article Snippet: The following antibodies were used for immunoblots (IB) experiments: rabbit anti-MOBP (1:2000; Sigma HPA035152) or mouse anti-TUJ1 (1:10,000; Covance MMS-435P).

Techniques: Expressing, Western Blot, Marker

Journal: eLife

Article Title: Exploring the expression patterns of palmitoylating and de-palmitoylating enzymes in the mouse brain using the curated RNA-seq database BrainPalmSeq

doi: 10.7554/eLife.75804

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-MOBP (Rabbit polyclonal) , Invitrogen , CAT# PA5-100618 , Dilution 1:1000.

Techniques: Recombinant